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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea canephora; Genetics. |
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Categoria do assunto: |
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Marc: |
LEADER 03034naa a2200445 a 4500
001 1004907
005 2015-01-13
008 2012 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Classificação |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
05/07/2022 |
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Data da última atualização: |
12/01/2023 |
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Tipo da produção científica: |
Publicação em Anais de Congresso |
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Autoria: |
FERRÃO, R. G.; FERRÃO, R. G.; FERRÃO, M. A. G.; VOLPI, P. S.; FONSECA, A. F. A. da.; VERDIN FILHO, A. C.; SENRA, J. F. de B.; COMÉRIO, M.; FERRÃO, L. M. V. |
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Afiliação: |
Romário Gava Ferrão, Incaper; Romário Gava Ferrão, Incaper; Maria Amélia Gava Ferrão, Incaper/Embrapa Café; Paulo Sérgio Volpi, Incaper; Aymbiré Francisco Almeida da Fonseca, Incaper/Embrapa Café; Abraão Carlos Verdin Filho, Incaper; João Felipe de Brites Senra, Incaper; Marcone Comério, Incaper; Liliâm Maria Ventorim Ferrão, Incaper. |
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Título: |
Conquista, cultivar de café conilon propagada por sementes para o Estado do Espírito Santo. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: CONGRESSO CAPIXABA DE PESQUISA AGROPECUÁRIA, 1., Vitória, ES. Anais 2021 : congresso capixaba de pesquisa agropecuária [recurso eletrônico]. Vitória, ES: Incaper, 2021. color. PDF ; 25,4 MB. E-book, no formato PDF. (Incaper, Documentos, 289). Pedro Luís Pereira Teixeira de Carvalho, Carlos Henrique Rodrigues de Oliveira, José Aires Ventura, Marcos Vinicius Winckler Caldeira e Romário Gava Ferrão, editores. |
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Páginas: |
p. 127 |
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Idioma: |
Português |
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Conteúdo: |
A cultivar ES8152 'Conquista' é propagada por semente, formada pela recombinação natural em campo isolado das 56 progênies superiores oriundos do universo de mais de 2.000 materiais genéticos de Coffea canephora do Programa de Melhoramento Genético do Incaper (clones e progênies
híbridas). Apresenta produtividade média de 74,10 sc. benef./ha em condições normais de cultivo, que é superior em 47,3% a cultivar propagada por sementes Incaper 8152 ? Robusta Tropical, lançada em 2000, e 11% a média das dez cultivares de café Conilon lançada pelo Incaper de 1993 a 2017. |
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Palavras-Chave: |
Base genética; Café Conilon; Conquista; Cultivar. |
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Thesagro: |
Café; Cafeicultura; Coffea Canephora; Melhoramento; Melhoramento Genético Vegetal; Melhoramento Vegetal; Reprodução; Reprodução Sexuada; Reprodução Vegetal. |
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Categoria do assunto: |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/item/4331/1/Anais-CCPA-127.pdf
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Marc: |
LEADER 02035nam a2200373 a 4500
001 1023870
005 2023-01-12
008 2022 bl uuuu u01u1 u #d
100 1 $aFERRÃO, R. G.
245 $aConquista, cultivar de café conilon propagada por sementes para o Estado do Espírito Santo.$h[electronic resource]
260 $aIn: CONGRESSO CAPIXABA DE PESQUISA AGROPECUÁRIA, 1., Vitória, ES. Anais 2021 : congresso capixaba de pesquisa agropecuária [recurso eletrônico]. Vitória, ES: Incaper, 2021. color. PDF ; 25,4 MB. E-book, no formato PDF. (Incaper, Documentos, 289). Pedro Luís Pereira Teixeira de Carvalho, Carlos Henrique Rodrigues de Oliveira, José Aires Ventura, Marcos Vinicius Winckler Caldeira e Romário Gava Ferrão, editores.$c2022
300 $ap. 127
520 $aA cultivar ES8152 'Conquista' é propagada por semente, formada pela recombinação natural em campo isolado das 56 progênies superiores oriundos do universo de mais de 2.000 materiais genéticos de Coffea canephora do Programa de Melhoramento Genético do Incaper (clones e progênies híbridas). Apresenta produtividade média de 74,10 sc. benef./ha em condições normais de cultivo, que é superior em 47,3% a cultivar propagada por sementes Incaper 8152 ? Robusta Tropical, lançada em 2000, e 11% a média das dez cultivares de café Conilon lançada pelo Incaper de 1993 a 2017.
650 $aCafé
650 $aCafeicultura
650 $aCoffea Canephora
650 $aMelhoramento
650 $aMelhoramento Genético Vegetal
650 $aMelhoramento Vegetal
650 $aReprodução
650 $aReprodução Sexuada
650 $aReprodução Vegetal
653 $aBase genética
653 $aCafé Conilon
653 $aConquista
653 $aCultivar
700 1 $aFERRÃO, R. G.
700 1 $aFERRÃO, M. A. G.
700 1 $aVOLPI, P. S.
700 1 $aFONSECA, A. F. A. da.
700 1 $aVERDIN FILHO, A. C.
700 1 $aSENRA, J. F. de B.
700 1 $aCOMÉRIO, M.
700 1 $aFERRÃO, L. M. V.
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Biblioteca Rui Tendinha (BRT) |
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